Teknologi Diagnostik Pada Penyakit Haiwan


Ketua Program
1. Profesor Madya Dr. Siti Khairani Bejo

Nama Penyelidik

2. Profesor Dr. Zunita Zakaria - Bacteriology /Molecular biology
3. Profesor Dr Faez Firdaus Jesse Abdullah - Ruminant Medicine 
4. Profesor Dr. Latiffah Hassan - Epidemiology/Veterinary Public Health
5. Professor Dr. Abdul Rahman Omar - Immunology
6. Profesor Madya Dr. Hazilawati Hamzah - Veterinary Clinical Pathology


Sinopsis:

      Laboratory diagnostics for veterinary pathogens have traditionally relied on methods detecting pathogen by culture or antibodies, using a variety of techniques such as neutralisation, enzyme-linked immunosorbent assay, agar gel immunodiffusion and complement fixation.
      Recently, veterinary diagnosticians have incorporate new molecular techniques such as the polymerase chain reaction and Western blot, and improved older techniques through the use of recombinant antigens and monoclonal antibodies. Although conventional diagnostic assays are still used routinely, new molecular techniques have broadened the scope of veterinary diagnostics and provide powerful new tools that enable the rapid and specific diagnosis of animal diseases.
      The ultimate aim of the research program is to establish diagnostics methods with quality control through effective isolation and rapid characterization of pathogens through utilization of molecular tools in complement with rapid conventional methods for fast and reliable detection of pathogens in infected animals for improvement of diagnosis of animal diseases.


Kata kunci :  laboratory diagnostic, veterinary pathogen, molecular tools

Research Highlights:

      • Technological skills of researchers with multidisciplinary background for conducting molecular methods and rapid conventional methods research towards production of diagnostic tools which give an impact to the society.

      • Multi-disciplines approach against animal disease and immunology evaluation assay in-vitro and in-vivo.

      • Accompanied by established research groups and competent researchers with proven achievements, together with over 500 technical papers, several patents, national and international of publications as well as research awardees.

      • Availability of suitable and conducive basic research infrastructure, equipment as well as facilities including Molecular Suite (PCR, Real-Time PCR, Image documentation), Proteomic Suite (2D-electrophoresis set, Image documentation, Western Blot), Domestic Animal Disease Suite (Conventional disease detection system, Microscopy unit, Staining apparatus, sterilization unit), Immunology Suite (ELISA reader and software) and Experimental Unit (experimental house with facilities of biosafety level 3 (BSL-3).

      • Availability of research funding to initiate Diagnostic Technology for Animal Diseases research projects for procurements of research materials and human capitals from various sources involving amount around RM 5 million.

      • Private and industry research collaborators or partnership international and local including with Dr. Pat Blackall, Research Manager, Principal Research Fellow, Queensland Alliance for Agriculture and Food Innovation, Dr. Rudy Hartskeerl, Head of Leptospirosis research and diagnosis, KIT Biomedical The Netherland, Mary-Anne Burns, WHO/FAO/OIE Collaborating Centre for Reference & Research on Leptospirosis, Queensland, Assoc. Prof. Dr. Mohd Mokhtar Arshad, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Prof. Dr. Abdul Karim Russ, Royal Medical Collage, Ipoh, Department of Veterinary Services, Zoo, Perhilitan, Global Veterinary Sdn. Bhd. And One Point Healthlab Sdn. Bhd.


        Current Projects:

        1. Development of local vaccine for Rimerella anatipestifier infection in ducks, Dana R&D Pertanian-MOA, RM 158,000.00.
        2. Development of Protein-based kit for ovine brucellosis, MOSTI, RM 97,997.00.
        3. Biomarker discovery for ovine brucellosis, RUGS, RM 126,000.00.
        4. Development of Protein-based Kit for Brucellosis in Ruminant, MOSTI, RM 191,000.00.
        5. Novel electrochemical product for a safe and healthy chicken production, MOSTI, RM 2,000,000.00


        Recent Publications:

        Y. A. Geidam, Z. Zunita, A. A. Saleha, S. Khairani-Bejo, A. Jalila and O. Sharina.
        High Prevalence of Multi-drug Resistant Bacteria in Selected Poultry Farms in Selangor, Malaysia, Asian Journal of Animal and Veterinary Advances,
        Vol. 7 (9), pp 891-89, 2012.

        Maged Ahmed AL-Garadi, S. Khairani-Bejo, Z. Zunita and A. R. Omar.
        Detection of Brucella melitensis in blood samples collected from goats. Journal of Animal and Veterinary Advances,
        Vol. 10 (11), pp 1437-1444, 2011.

        Mohamed-Hassan, S. N., Bahaman, A. R., Khairani-Bejo, S. and Mutalib, A. R.
        Serological prevalence of leptospiral infection in wild rats at the national service training centres in Kelantan and Terengganu. Tropical Biomedicine,
        Vol. 27(1), pp 30-32, 2010.

        Ridzlan, F. R., Bahaman, A. R., Khairani-Bejo, S. and Mutalib, A. R. (2010).
        Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia. Tropical Biomedicine,
        Vol. 27 (3), pp 632-638, 2010.

        Andrew T. Slack, Siti Khairani-Bejo, Meegan L. Symonds, Michael F. Dohnt, Renee L. Galloway, Arnold G. Steigerwalt, Abdul R. Bahaman, Scott Craig, Bruce J. Harrower and Lee D. Smythe.
        Leptospira kmetyi sp. nov., isolated from an environmental source in Malaysia, International Journal of Systematic and Evolutionary Microbiology,
        Vol. 59, pp 705 - 708, 2009.